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Title   인체 위암 세포주에 retroviral vector를 이용한 p53종양 억제 유전자의 형질도입에 관한연구 ( A Study of REtrovirus0mediated p53 Gene Transduction Into Hman Gastric Cancer Cell Lines )
Publicationinfo   1997 Jan; 029(05): 754-765.
Key_word   Gastric cancer cell, Gene therapy, Retroviral vector, p53 gene
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Abstract   Purpose: The development of new therapeutic modalities such as gene therapy, which still requires further investigation, is clearly important to improve the prognosis of gastric cancer. This study was conducted to evaluate the effect on the growth and the tumorigenicity of retrovirus-mediated p53 gene transduction into gastric cancer cells. Materials and methods: Human gastric cancer cell lines were cultured and their DNAs were analyzed to evaluate the p53 status with PCR-SSCP(polymerase chain reaction-single strand conformation polymorphism) and DNA sequencing. Retroviral supernatants were obtained from each producer cell line, PA317/LNCX and PA317/LNC/p53, after construction of retroviral vector LNC/p53 containing human p53 cDNA and producer cell line PA 317/ LNC/p53. To investigate the effect of retrovirus-mediated p53 gene transduction in human gastric cancer cell lines, the in vitro growth rates and in vivo tumorigenicities of the N-87 cell line having mutant p53 and the YCC-S-2 cell line having wild-type p53 were compared before and after infection with LNC/p53 retrovirus. Results: The following results were obtained: 1) The growth inhibition of N-87 cells after p53 transduction was signficant when compared to that of the parent N-87 cells. The growth of the p53 transduced YCC-S-2 cells and the parent YCC-S-2 cells was not different. continue......
저 자   김주항(Joo Hang Kim),문유선(Yoo Sun Moon),신동환(Dong Hwan Shin),공수정(Soo Jung Kong),라선영(Seon Young Rah),김수경(Soo Kyung Kim),정숙정(Sook Jung Jung),정현철(Hyun Chul Chung),노재경(Jae Kyung Roh),민진식(Jin Sik Min),김병수(Byung Soo Kim),송재진(Jae Jin Song)